LOCUS BG070238 815 bp mRNA linear EST 12-MAY-2010
DEFINITION H3085B09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3085B09 3', mRNA sequence.
ACCESSION BG070238
VERSION BG070238.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 815)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG070238.1.
Other_ESTs: H3085B09-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3085 row: B column: 09
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..815
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3085B09-3"
/db_xref="taxon:10090"
/clone="H3085B09"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 246 a 203 c 123 g 243 t
ORIGIN
1 aaatagccag cttgcatttt agacgattta ttgcataaaa tagggtatgt atctgaatat
61 atttcatgct actgtggaaa aaaagtctga actgctgacc ctgctgggaa aaagactagc
121 tgtgcgtgac actccgtaca cagtgtaagc atctcttttc accaacctct ccaaatacct
181 cagtagtaca cggcttctgg ctgcaaattc ttgcaacata accactttta aaaatgtaca
241 ttaacttggt aaacaccagg gtacattgcc aaataccttt cttgtatgac tctgtggaca
301 ctcaagagtg tttccttggg catggtagcc atccataggt gacaagacaa catatagaac
361 ttcacacgac acggttccct catccatatg ttaaacattg agatagtgca ctcttaaaac
421 ataccatcac aacacaaatc acttcctctc actcgtctaa cttctccaac tttaaaacct
481 tggcacacca aacaggagtt ttgactcata tttttaaaac aaaataagtt tcacaccttt
541 ggagaaaaag caaactggct gattcttccc catgataata tataataatt cttccctctt
601 tattcagtta attataaaac aggccactgt ggcctcattt atccgcagtc tccttttgga
661 atcaaatatg tcaaaaatac ctctgtgggt catactttca tgatgtcttc aaagcagcct
721 tttttagttt tctctgcaca ccacttagcc aagatcatac catctctagc ccttgtctcc
781 atccaccccc tgccccaagg tgaacactgt ctgtg
//