LOCUS BG070144 688 bp mRNA linear EST 12-MAY-2010 DEFINITION H3084B09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3084B09 3', mRNA sequence. ACCESSION BG070144 VERSION BG070144.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 688) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG070144.1. Other_ESTs: H3084B09-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3084 row: B column: 09 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..688 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3084B09-3" /db_xref="taxon:10090" /clone="H3084B09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 195 a 156 c 145 g 192 t ORIGIN 1 caaattaaaa agaattggat atgagtattt gagtctgtgc ccatgatgct ggagggtgtg 61 agtcagatcc ccgggagctg gaattatgtt gtgacccatt tgctgtgggt gctgggagtt 121 caagcccacc ctctgtgcga gtagcaagtg ttcccaacca cttccatccc ccatgtttgg 181 cttttatttc atatattcaa tttaatttaa atcccagagc tgtaagaaac atccgtttat 241 tgtctagtac tgtctctgac aatcaaaata atattgacta tttcctccaa ttgcttgaaa 301 agattaatcc tgatttacaa aaaaatattt tttgacccag ggtctcttca caagcccaag 361 ttggactcga acctgcaatt ttcctgcctt tgcctctcac atgctgggag acaaatgaaa 421 gaacttaggg aagcgcagaa aaccatcacc actacaggat cgtcccagaa ggcagaatct 481 cgcccacaga gaaactgtaa ctgaaaagta cagaagttac tattcttgaa aagccagtaa 541 ctcttttgat tctgtttaag gatggttttc ctgagccagt gaaatggctc agtgcgcaaa 601 ggggcttgct gccaaatctg ataacctaag ttcagttccc ggaacccaca tggactatct 661 gtgccacggc atgtatgaga ccgcgcgc //