LOCUS BG070100 597 bp mRNA linear EST 12-MAY-2010 DEFINITION H3083F07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3083F07 3', mRNA sequence. ACCESSION BG070100 VERSION BG070100.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 597) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG070100.1. Other_ESTs: H3083F07-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3083 row: F column: 07 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..597 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3083F07-3" /db_xref="taxon:10090" /clone="H3083F07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 121 a 162 c 156 g 158 t ORIGIN 1 gagacagggt ttctctgtgt agccctggct gtcctggaac tcactctgta gaccaggctg 61 gcctctaact cagaaatccg cctgcctctg cctcccagat gctgggatta aaggcgtgcg 121 ccaccacggc cagctacaat ttatttttat tatttattgt ttggagaagt catgcatgtg 181 gaagcatgaa gacaaccggc aggactggtt ctcttgttct tccctgtgag tcctgggaac 241 tgaaccagtg gtcaggcttc acagcaagcc ccgttattgg ctgagcatgt catcagccgt 301 gcagttggtt ttcataccat gctttttggg gtttgaggta acagttctga ggtctggtgt 361 actgggcagg cactcactcg cccctatcca tgccctctag gaagctgttt taagctaggt 421 gattgcggcc ctgctcaggc cgtaagtgca gcagatggcg acagagagcc cagcttcccg 481 ggctctgtga tgcctccctc atctgtacaa cagagactgc tcctctccct gtgctcctta 541 agtaagctca acagtgagac aaatggctca gctgttagag cactggccgc ccttgca //