LOCUS BG070086 622 bp mRNA linear EST 12-MAY-2010
DEFINITION H3082F03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3082F03 3', mRNA sequence.
ACCESSION BG070086
VERSION BG070086.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 622)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG070086.1.
Other_ESTs: H3082F03-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3082 row: F column: 03
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..622
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3082F03-3"
/db_xref="taxon:10090"
/clone="H3082F03"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 146 a 165 c 176 g 135 t
ORIGIN
1 ctgaagtgtg tctctttatt ttaacagcct tgcaggatca ggtaacagta cagggttcag
61 aggtccatta gcccaggctc gggcaatagc tcctgtctga ctatccagag cactgagccc
121 tcgacaaagc ccaggtcaga catcagcccc aggggtaatc cagatcctgg gcataagtag
181 gacagtcaaa acctcctcca gtgtaaccaa acaagctctc atgaggcggc ttgtttccct
241 ggcctggctg gctccttccc ccgaagcttt tgcctcaggt agataagcag cactcgggaa
301 tgtatccctt ttctgataca tgaactttcc ccagtctgga acatggaata ctagcacaac
361 agggggcgtg accacaacct attagcatct gggaggctcc cattgggcat caagtggcga
421 cacaaagcag ggctgtctgc tcgggctgag agctgggcac acagagtggc caggcggcag
481 ggtgtgccgc aggctctgaa ggtgggtggc ccttatggta gagaaaccag aaggtctgga
541 agagttgctc atcatccctc atgcggtaga ccaggttgtg ccaggaggca ggcatcgcat
601 ctgggagtcc ataggtttct cg
//