LOCUS BG069997 470 bp mRNA linear EST 12-MAY-2010 DEFINITION H3083E01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3083E01 3', mRNA sequence. ACCESSION BG069997 VERSION BG069997.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 470) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG069997.1. Other_ESTs: H3083E01-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3083 row: E column: 01 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..470 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3083E01-3" /db_xref="taxon:10090" /clone="H3083E01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 98 a 128 c 154 g 90 t ORIGIN 1 gggttaaaag gctttattag ggaaacatac aggggcaagg accatccttg ggagacctca 61 ggacgctgtc ctccaggttg ctgggcaggt acagtcgcca ggagcccctg ctcagaagca 121 gctgacctgg gaggcaggag gaggggtggc ttctactagt gaaactgcac cctactcccc 181 agtattaagc tgtggccctg gcacccattc ctccccaggg atattggtag caggaggcag 241 tgacgcatgc agcaggtctc tctcaggcca gggccaccct gctgcttccc acggacacag 301 tgctgctcac cagtctccac tggggcctcg tctgggactg tgggggaaga gcatgcagcc 361 tgcgaacgca ttgcccatga tgcggcagag gcccttgagg gagtcacagt ctctgttggt 421 gaagtggaac tgggccagct gagagtttcg gaacagggga cccagtgtgg //