LOCUS BG069931 560 bp mRNA linear EST 12-MAY-2010 DEFINITION H3081F10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3081F10 3', mRNA sequence. ACCESSION BG069931 VERSION BG069931.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 560) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG069931.1. Other_ESTs: H3081F10-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3081 row: F column: 10 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..560 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3081F10-3" /db_xref="taxon:10090" /clone="H3081F10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 152 a 123 c 135 g 150 t ORIGIN 1 aaaaatgtaa tgctttagtt tatttcacaa cacttgtctt ttctcccaat aagtcagaaa 61 caatgtaggt tggcatgaca gatccttcat ccttatttga tggatgtcca gtgtatgtgc 121 atgtctgaca gaacaagatc ccataggtca atggatgtag acgattatat tccattcact 181 ccagggcacc aggagatgag catctgcatc tctgtgagct ttggtgtgtg ggtctctctg 241 caggcttctc ttagaggtga ctctgcacct ggtgtcatcc tggacaccca gaacaggagg 301 aatagagtgg tagctttata aatattggtg gtatgatgga cagtgcaagg tcatctctga 361 aaatgtgaca tctcactctc gcccacagta gtgacaatac tgagtgggaa tagaatcact 421 caggggactg gagagatggc tcagtggttg agaacactgg ctgcttcagt acctgggttc 481 aattcccagc acccacatgg ccactcaaac tatctggaac accagttcta ggggatctga 541 cacccgacag aacacagatg //