LOCUS BG069832 785 bp mRNA linear EST 12-MAY-2010
DEFINITION H3080F03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3080F03 3', mRNA sequence.
ACCESSION BG069832
VERSION BG069832.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 785)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG069832.1.
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3080 row: F column: 03
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..785
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3080F03-3"
/db_xref="taxon:10090"
/clone="H3080F03"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 217 a 189 c 167 g 212 t
ORIGIN
1 ctagtcttct aaatcattta tttttccata tttatatttt taaataaata aggatatatt
61 caattgtaaa ctctttctgg ccaaagagtg aaccttggaa gaagtctgca tgcatcttgg
121 ggcctcagga caccccaggg ttttaaaagg acatagaagt caaaggccaa acatttggtc
181 tcagctgtca cctgctactc agtcccatcc caggtctttc tgctaggtac tgtccctgct
241 cacagccaga agcagcaata gctggaaaaa gtgacgactt gggactccag tgaaccataa
301 taaaccaacg aataaggtga aactcaacag catcagcttc tccaatatgg aaagtcacct
361 gcttcaataa accactgtga actgcaaatt ctactgggaa gacagccttt tctctcccag
421 acaggctgcc ctggacttca taatcctgcc tcaactattc ctggcttgaa taaggtttga
481 tgtggtaaga gttccagaca ggtttctctg tagccccggc tgtcctggaa ctctctgtag
541 accaggctgg cctcgaactt ggagaaccac ctgcttctgc ctcctgaatg ctgggattaa
601 aggcgtgcac taccacagcc cagcagtttg gtgcttcttt gtggacattc tctggcacct
661 tagaaaagac tgaggtaatc ctggaacttc ccagtttggc ccaagatcaa gatcataaag
721 tcttaggggt cggggggcaa gtacagccat agcaatttac atttagatag ctttacattt
781 agagg
//