LOCUS BG069790 725 bp mRNA linear EST 12-MAY-2010 DEFINITION H3080B08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3080B08 3', mRNA sequence. ACCESSION BG069790 VERSION BG069790.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 725) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG069790.1. Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3080 row: B column: 08 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..725 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3080B08-3" /db_xref="taxon:10090" /clone="H3080B08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 178 a 210 c 179 g 158 t ORIGIN 1 ctgtgttggc aatttaattt tacaaaacaa ctctagccac cacattttct cctctgtcca 61 tcatttccct tttacaggaa gttacaaaaa catcaggggg ctttaccatc tctacaaccc 121 aaacaagaat cagaagtgac tcccaaagcc ggccggcaga gaataaagac aacgtaagaa 181 cagatcacgg ttatagcaat acaggatcgc gacattgatt tacagtgcag tggttgtacg 241 ttcaccagag ggaattctga gccagagact aaagtaaaag cacctgcccg cttgctgcca 301 ggaggcccag cgcaagatca cagcaacagg gcaccaccac gcagtaaggc ttttctggcc 361 ccgaggatgg agagggcgct ctgctcggcg agggcccggg gcctcacttg gagtcttggt 421 ccttctcatc taccagggca gagcggatac ccagcttttt cagttcttcc actaggtccc 481 cattctgatg catctgcaga aggatgtcac agccccccac aaactcgccg ttgaggtaca 541 cttgcgggat ggttggccag ttggagtagt ctttaatacc ttgcctcagc tccgggtcgt 601 ccagcacgtt gtaggccgca tagtcgcgaa caccgtgcag ccgcagaatc tgcaccacgg 661 cgttgctgaa gccgcactgg ggctgctcgg gcgtcccctt gaggaacacc accaccttgt 721 ccttc //