LOCUS BG069763 739 bp mRNA linear EST 12-MAY-2010
DEFINITION H3079H03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3079H03 3', mRNA sequence.
ACCESSION BG069763
VERSION BG069763.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 739)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG069763.1.
Other_ESTs: H3079H03-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3079 row: H column: 03
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..739
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3079H03-3"
/db_xref="taxon:10090"
/clone="H3079H03"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 194 a 186 c 132 g 227 t
ORIGIN
1 aagatttatt tatttattta ttatatgtaa gtatttttgc atgtggcaga ggggcggggc
61 ttattatttc atctcatact ttcaggtagc aaatgtccag cattttgaga agcaaggaca
121 ggaactatag cataagaaag aaccgtgcag gaaagcttcc tgcttgttgt ctttctccct
181 agcctctgtt cagttagttt tctacatgta tatgtaattt attactatta ttaatattaa
241 cttgaggctt tcataccagt cgctctcctc catcttctcc cagatctact cccattccaa
301 atccacttaa ctttgcatcc ttatgtcttt tcaaacccag ccagccccct caatgctgtc
361 tgtactctct tagctgtgca atcatccact ggaacactag acccaccagg ggacacaccc
421 ttaaagaaag ccaactccct ctcccagtag gcgtcactaa ccagttgctc ctcagctaat
481 ggtgagactt catgcccaac ttttctggaa aaccctctat tgctcagttg tcttaagttg
541 tcagaatatg gaccacttca gagacatgtc agtagatttc ccaattacag cccaaggatt
601 ccccagttta cacatactcc aggtctctgt ggtataagga tatcccaagt tctggcttag
661 tttcatcaac aatgggcatg cataacgtgc ccacagctgc cagtgtgtgt ccacaagaca
721 gatcggggtg catgaatat
//