LOCUS BG069650 766 bp mRNA linear EST 12-MAY-2010 DEFINITION H3078F05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3078F05 3', mRNA sequence. ACCESSION BG069650 VERSION BG069650.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 766) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG069650.1. Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3078 row: F column: 05 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..766 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3078F05-3" /db_xref="taxon:10090" /clone="H3078F05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 236 a 180 c 163 g 187 t ORIGIN 1 acctctcaat tacgtaatgt gagtctgtct aaatctctca gtcaattatg cacaagccca 61 acctgccttc ctgctgcttt tggaacacta ggcagctccg cccacccaac gactcaaagt 121 aaaatattaa gagatatgaa cagctgcact tatccgccac accaagaata gctttcagga 181 aaccaagctc cctttggaaa actgcattta gtaaccccac tcagctgcag agagtactcc 241 taattaatga ggaaaatgcc tgcatgttct ttaagatatt tatagaatac atattttctc 301 ctagaagaca acaaattaaa tagtggtgtg accatttata ccctaggagt tcgagctgac 361 actgcttgta gctctcagaa caacaaagtg ctaaagcctt gccactagct cactctccac 421 acttctgcac ccagacagga agttcgtaag cctgtcccca ctctcctttt ctggctgctt 481 ttcatactca cctgccctac cttggcccca acctctgatc tatgtggaca actatgggtt 541 taaacaattg taataatagt tgagtagtgg tggtatacat ttttaacccc agcacttgga 601 aggcagaggc aggtggatct ctgtgagttt gaggccagcc tggtctacaa agcaagttgc 661 caaggcaact cagagaaacc ctgacttgaa aaacaaaaaa ttagtggagg actgagaaaa 721 agagggagag gaggaggaga aggaggagga agaggaggag gaagga //