LOCUS BG069637 721 bp mRNA linear EST 12-MAY-2010 DEFINITION H3078E04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3078E04 3', mRNA sequence. ACCESSION BG069637 VERSION BG069637.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 721) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG069637.1. Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3078 row: E column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..721 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3078E04-3" /db_xref="taxon:10090" /clone="H3078E04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 210 a 182 c 174 g 155 t ORIGIN 1 acttaaagtt tttactttga gacatcatgg ggaaaaaaat acagggatac actgaagaga 61 gagacatgat cacagaaaaa aaaaaaagga tagaatagaa gcatcggtgc ctgtcagcca 121 gtgttgaatg tgaagctctg agagtatgcc atgctacagg ggctcaaggg actcctcacc 181 ctctccttca acaggatcca caccaacctc ctcatcaacc ttctccaggt cagccatgtc 241 ctcatcggct tcagagaacc ccgccccctt ctgcattgcc ctcacccaca gaccactgca 301 caaaagaaca cagggtatat atcaggtcaa acatacggtc taggcaagcc caggcttcag 361 cagtggctgt ggtgttgctc agccaggtcg ctttcaggta ccagactaga aggcttgtaa 421 ttaatgccaa tcttgaagcc agtgtggcac cagtccacag actgcatgtt gtgcttagtc 481 taaatggtga cagtggcagc attggcatct gtggctacca tatcactaag gtgcagcatg 541 cagaaagtca tgtatttacc acggcaaggg tcacattcca ccatcgggtt ggctggctca 601 aagcaggcat tggtgaactc tgctacagaa agccacccat ggtaagattt ctcagcagag 661 atgacaggaa aatctgtggc cagcaggaaa tggtgcagag gtcacacacc agggtagcct 721 g //