LOCUS BG069636 703 bp mRNA linear EST 12-MAY-2010
DEFINITION H3078E03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3078E03 3', mRNA sequence.
ACCESSION BG069636
VERSION BG069636.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 703)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG069636.1.
Other_ESTs: H3078E03-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3078 row: E column: 03
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..703
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3078E03-3"
/db_xref="taxon:10090"
/clone="H3078E03"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 193 a 176 c 126 g 208 t
ORIGIN
1 ctttgtcatt tcttctttat ttagcatata cagtcttatg aaaagtttgc aataatatga
61 acactgacgt ctcctactgc aaggacagaa gacaaaacct aacagtagac caagaaaaga
121 agatccgtgt aggattaaga cccaaaagca cggtagcatt tatttagaag tcagcggttc
181 agcaagtgtt ctgaggatgt tctccatagg tggagtgttt ctcgtcagtg catgcacagc
241 agcaagagaa actaatattc taaatagggg cccacaaaag caaatgtcag acccaccctc
301 ccaactatca ccactacaca tccttcacat gaaaccctca cacagtgtta ggcgaattct
361 agaaactact aagatcaggt cgatgaaccc tgctcggctt aggcgggaca gacaactcct
421 ctcaaaggtt acattaatgg ctgtcatcat tttgtctaca ctgtaccact gacacataag
481 gactcaggaa gagaaaaact ccatcatttc ttttccttct tgagtgctgt tcttttccct
541 cctttggaag gtttgcagta ttttgcttcc atttgagcca gaaaactgtc catttccttt
601 tgccgatcct tttgtctgct ctggatgagt gctttcaagt tatccactcc ttcttccagt
661 cccagctcct ttctgctcaa ctctgcttct ttagcctctt cct
//