LOCUS BG069629 773 bp mRNA linear EST 12-MAY-2010
DEFINITION H3078D08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3078D08 3', mRNA sequence.
ACCESSION BG069629
VERSION BG069629.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 773)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG069629.1.
Other_ESTs: H3078D08-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3078 row: D column: 08
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..773
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3078D08-3"
/db_xref="taxon:10090"
/clone="H3078D08"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 226 a 165 c 176 g 206 t
ORIGIN
1 cgagacaggg tttctctgtg tagccctggc tgtcctgaaa ctcactctgt agaccaggct
61 ggattcaaac tcagaaatcc acctgcctct gcctcccaag tgctgggact aaaggcatgt
121 accactactg cccggcctac atcacctctt gataggatat tttatgacag tacatgaact
181 actgcacaga gcgctgcccc accaaacact tcttttagca ggaagaagtc atgtcttggt
241 gaagtgacaa gtgtgagagc actgctcaag agaaaacttt acactaggtt cagagggttc
301 taaagacaga gagacttccc aaccacccct agaatattct gaaagggaag gagactcaga
361 gattctacct ctgtaatatg aaggccctct tagacccttt aaagcctaga gaagtcatgg
421 acaagtcaca tatactagtg ccctttgact aaaaaaagta cactagagta ggcatgaaat
481 cgtgacattg tttctgtgtt ttgttttgtt ttttaaaagt ttaagtgcat gttaatggtt
541 agcttcaatt atcaactgga caaaacttag tatcacctgg gaagaaaggc tcaatgaacg
601 actgtttaca ttggattggc ctttgggctt gcctgtggtg ggttgaacta attatgttaa
661 ctgatgtggg aagacctagt ccactgtggg tggcaccatt ccctatgcag ggtattctga
721 aatgtatggg agtgaagaaa tcaaccgtgc acagacaaac aaacaagctg tga
//