LOCUS BG069599 618 bp mRNA linear EST 12-MAY-2010 DEFINITION H3078B01-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3078B01 3', mRNA sequence. ACCESSION BG069599 VERSION BG069599.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 618) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG069599.1. Other_ESTs: H3078B01-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3078 row: B column: 01 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..618 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3078B01-3" /db_xref="taxon:10090" /clone="H3078B01" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 205 a 125 c 101 g 187 t ORIGIN 1 gcttattcaa aacagattta ttagaaccta tgagaaatta taatggtaac agtggtggta 61 gaagataata gcaggctact ggacctcccc aagaaagaga tacagcttaa tagtgataac 121 agtctaatac aaaagtactc caagtttagt cagatttata cttcataaaa atatatccat 181 atactctaga acagtaaggg gagagccaaa aggtgggtgc catctgcagg gagaatgaaa 241 cagagacctc ctattcaagt ccattatgat ctttataatg tcctcactct ttatcccctt 301 ccctacttct agtgtccctc atactcagac agtgctggaa tgcagcccta caattttcag 361 atacaattgc tgttaaaggg tgtgtctgta ctgtagacat taggctgaaa ataaggtcat 421 ggttttccat ctcaacacaa ctacctcaaa tcaggagaat ttgactgaca ttcaaagaaa 481 atgaaataat tatctttcca aaagtacatt ccaaaatgct tgaatacata tgttctataa 541 ctctaatgaa tctcttgaga cctgccctcc atttatcaga gcccagcaat gacctctcct 601 ttttttttta attttttt //