LOCUS BG069526 458 bp mRNA linear EST 12-MAY-2010 DEFINITION H3077C11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3077C11 3', mRNA sequence. ACCESSION BG069526 VERSION BG069526.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 458) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG069526.1. Other_ESTs: H3077C11-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3077 row: C column: 11 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..458 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3077C11-3" /db_xref="taxon:10090" /clone="H3077C11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 121 a 105 c 119 g 113 t ORIGIN 1 gggatttaaa attttttatt tcaattgaaa attaattttg ggagtaaaat aagcatgggt 61 aaaagtcatt ggattctttc agacaattta aagcaacaac acggggagag gcgtgaacgt 121 tttattgcat gcttgcagga cgccatgtcc tgtcataggt gctctaccca atcagctcac 181 tggatcctca caacaaccct gtgacgtgag ttcctagcct cacagtccag gctcagaggc 241 cgtgtaaacc tggcaatgtc ctgcaaccca taaaggatgg agtcaggcca ggtgcctctg 301 gaccctcctg ctgtaaggaa ggctcctgaa ggtgagtttc ttcacgtcat gtccatcccc 361 cagcttgcca cacatctttc tggagggcta agaggagggg tgagagtata agtggccatc 421 cggggaacat ggaggagtgc catctggcta cagagacg //