LOCUS BG069467 712 bp mRNA linear EST 12-MAY-2010
DEFINITION H3075G03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3075G03 3', mRNA sequence.
ACCESSION BG069467
VERSION BG069467.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 712)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG069467.1.
Other_ESTs: H3075G03-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3075 row: G column: 03
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..712
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3075G03-3"
/db_xref="taxon:10090"
/clone="H3075G03"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 154 a 191 c 159 g 208 t
ORIGIN
1 aaaaaatgta gtagtttatt gaactcggac aaatggccac aaggcccaca gcaccgaggc
61 ttgcccggat agaggctcaa agctgctctg accagtgcct cttcatcaag atgctttctt
121 ttgcttcttc ttttcatttt cttctttctc cttctcaatt tcagcaacat acttctcaat
181 ttcttcagga tttagaatct tgagcggctg atcccgcctc atgacggcaa gttcgatgtt
241 tttgccacct gactggacca cctctaacag tgccttgatc acaagtttga tggtcagatc
301 atctgtttca atggcatcat ctgtgtagtt ctctccagaa attcacgcac tgacttggcg
361 ccccggccta tggcattggc cttccaagca tggtatgtgc ccgaggggtc agtctgatag
421 agtctgggag tgccatcaaa gtcaaaaccc acaattaggg ccgagatacc aaatggcctg
481 cgcccattgc tctgtgtata acgctgcttc agactcgcga tgtagcgggt gatgtactcc
541 acagtcactg ggtcctccac tgtcagccgg tggctctggc actctacccg ggctctgttg
601 atgactatcc ttgcatcagc ggtgagacct gcaaaggcca tacagacgtt atcgtccaag
661 gcgcagattt ttcgtactgt tctttcatct tgtagcttgg ccactgattt tt
//