LOCUS BG069459 726 bp mRNA linear EST 12-MAY-2010 DEFINITION H3075H05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3075H05 3', mRNA sequence. ACCESSION BG069459 VERSION BG069459.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 726) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG069459.1. Other_ESTs: H3075H05-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3075 row: H column: 05 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..726 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3075H05-3" /db_xref="taxon:10090" /clone="H3075H05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 187 a 189 c 167 g 183 t ORIGIN 1 aggtttttcg agacagggtt tctctgtata gccctggctg tcctggaact cactctgtag 61 accaggctgg cctcaaactc agaaatccgc ctgcctctgg ctccagagtg ctgggattaa 121 aggtgtgcgc caccaccgcc cagctggagc tgtcttaaag ataaataact actcatgaaa 181 ataccttgaa aaatgtaaca tcttagataa gtagacgaca ctgttgttat aattgacttc 241 acataccaaa gtgatcccag tcttcacgaa ggttctgaat ttccaactgg ttcttgcctt 301 cagtgaaaat aggtttgggg ttcttctcaa agcctccaga caagatgcca ccctgccagt 361 tcctaatgta aattcttcca tcagcatcca caatggttgg tgtgttgctc tgcagagggg 421 tgtcccaggg acgagtcaga aggtagaagt gttcacaggc gtgtagcggg atactaagcg 481 gctcctcgtt ggacagaccc agctcatatg cccactgccc ggcacagttg acaaaatact 541 ggcattcgat ctgtcctttg tctgtctcca caccagtaac ttgacctttt ttgatcagta 601 cgtgaagaac acttgtccgg tcataaatct gaacaccatt ctgggaggca gcacttgcca 661 gggccagagc cacatcagca gaggacacta ctgcatcctc aggaacgtac atggccccga 721 ccagat //