LOCUS BG069456 744 bp mRNA linear EST 12-MAY-2010 DEFINITION H3075H02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3075H02 3', mRNA sequence. ACCESSION BG069456 VERSION BG069456.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 744) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG069456.1. Other_ESTs: H3075H02-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3075 row: H column: 02 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..744 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3075H02-3" /db_xref="taxon:10090" /clone="H3075H02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 182 a 153 c 220 g 189 t ORIGIN 1 atttaaaatt tttttttatt ttttaaatat catttatttt actttacttg gatgtgtgct 61 tgagtgtgtg tatgtgaacc atgtatgtgc agaagcccgt ggaggtcaga tgagagcatt 121 ggaccccctg gaactagagt tataggtagt tgtgagccac cacctgggtg ctgagaactg 181 aaccccggta ctctgcaata gcagattgtg caccaccggg ccctatctct ccagacccta 241 acaaatgtat ttatgtgtat gggtttgtac acatgtgtgc agtgccctca ggggccagaa 301 cggggtactg gagcctctgg atctgggatt acagacagtt gcaggtgttg agaactgcac 361 ttgggtcctc tccaagagca gggagcattt ttaattgctg agccgtctct gcagctcctg 421 ggggtctttc taatggaagc cgaggggtaa atttgcctgt atctggatgg gagcctttgg 481 aaagcatgct gggaaattcc tcctctggag cagcagctag ctggaaaggg agcactgagg 541 ctggaacgtg gccagcatcc ttggacactg gggccagcat ccatgaggag agataggctg 601 cttctcaggc tctcgggagg cctggatctg gctgtgtagc tgctggaggg acaaatgtcc 661 agaacaggaa ggtcaatgtc caaaatagga agagcaatga gcccaagaac tggccagaga 721 attgaggacc aggtgagaga acag //