LOCUS BG069415 662 bp mRNA linear EST 12-MAY-2010 DEFINITION H3075C10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3075C10 3', mRNA sequence. ACCESSION BG069415 VERSION BG069415.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 662) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG069415.1. Other_ESTs: H3075C10-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3075 row: C column: 10 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..662 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3075C10-3" /db_xref="taxon:10090" /clone="H3075C10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 209 a 165 c 131 g 157 t ORIGIN 1 aatctgaaca gaaaataagt acacaagttt gctaaggaga catggtaaaa ctgaatgtga 61 tgcatttatg gaatcaatac atcagggtta tcttggtagc cagtgattgt gagttatgaa 121 gaaacaagac cattaaatgc ccctttcaga gcccactcaa tcaaaccgat ttcacaacta 181 cgtatgatca cgttcactct cagccctcct ttgtggaaac aacagttagt ctctaatggt 241 tccggggagc cagagccgat acaggagtag ttgtccagga gtcagagacc gttcctagga 301 aatgtcattc acccatgtgc atgagaatgg cttagcagtt ttacaaactg aaaccacaga 361 caaggacggc agcagcgttg catgctcagt acactacgtc atatagtgcc tctacactca 421 tgtggcgctc accattatac aaccacattt ctgatgagac tgtccatctc ttcacaaaat 481 ccaaaatcct cctaagacct tgagcttcac acaggagatt atgtctgaac attccaaaca 541 ctggtcagac tggtacaaca ggtagcaacc acaggctgta agcacggctg catacacact 601 gttgtacacc acatacacac tgctgtgcac catgagaacc ttcaaaacaa ctcagtccag 661 cg //