LOCUS BG069401 750 bp mRNA linear EST 12-MAY-2010 DEFINITION H3076H10-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3076H10 3', mRNA sequence. ACCESSION BG069401 VERSION BG069401.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 750) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG069401.1. Other_ESTs: H3076H10-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3076 row: H column: 10 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..750 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3076H10-3" /db_xref="taxon:10090" /clone="H3076H10" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 173 a 184 c 178 g 215 t ORIGIN 1 ccttcaagtt tttgtttggt tggttggttt tattggcaag catcagtgcc gcattaccgt 61 cgattcccca ggcttcatcg tgctgcggtg agaccactgg aaagcacggt gttctcagca 121 caaggctggg actgctcctt caccaccggg tagaaatatg ggtgctccat ggcctctttg 181 gcggtcaatc tctgttgatg gtcgtaccgc agagcttgtc aagaagatct agggcctccg 241 ggctgacaag gtgcctgttc tcactatgga taaagttttc ccagcgcttc cgtgaatgtt 301 gtcccaggat atcattgaag tgtggatcta ggtctatgtg gtacttcttc agataaccat 361 atagttcatc tgtccccaga accttggcaa ttcgaacaag ctggtcatag ttgtcctgcc 421 cgtggaagaa tggctccttt cggaatatca tgctcgctaa catgcagccc aagctccaca 481 tgtccaagct ataatcatac atctgatagt ccacaaggag ctctggtccc ttgaagtacc 541 tcgaggccac tcgaacattg tactcctgag caggatgata gaactctgcc agaccccaat 601 caatcagtcg gagctttttt tgttggtgat ctatcatgac attgtgaggt ttcacatccc 661 tgtgcatgat tcccttgctg tggcagtaat ccagagcttt aagtagttca tacatataaa 721 accggatatc aaagtcagtc aggatctggt //