LOCUS BG069397 825 bp mRNA linear EST 12-MAY-2010
DEFINITION H3076H06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3076H06 3', mRNA sequence.
ACCESSION BG069397
VERSION BG069397.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 825)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG069397.1.
Other_ESTs: H3076H06-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3076 row: H column: 06
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..825
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3076H06-3"
/db_xref="taxon:10090"
/clone="H3076H06"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 268 a 189 c 166 g 202 t
ORIGIN
1 aagcggaaaa aacatttatt tctatcaaat tcaatacaaa tcaatcttca agcaaggtaa
61 caaaacaaaa caaaaatcaa tgaatacaat gaatagttag gatagcagat acagaacaga
121 ctctgaacta agcacatata cccaaatcaa gaggaacacc aactgtcact gaagaaaagc
181 tgacgtgcca gctccaggga aacttaagcc aacactgagt taaagactgg cttgaacttg
241 ggggatcaca gtgaaatgcc atcctagctc aacgtcgtcc cccagagatc ggtccagctc
301 aaaaaaacaa aattcgcaaa ttcagtagta caagttctca taagactaga gaagccttga
361 gctctaccat ttcttgcagt ccacacagag cctggggttt tcaatttttc ttcccttttc
421 ccaaatccca gagagaaggt gcggtactcc ctgcaaaggt tttaaacagg agccttttat
481 taaaagacac tgtgcttcct gtctgtcact agagggagtc aggatggagt cctgggcaat
541 ggcttcctgt ctccctcaca cccaaccaca taaagaacag tcaaaccaag gagtgccctg
601 aggcagttga tggcatttgt tctggcaaga attgtgcagt ttgccacacc aaaaataagg
661 gtaaccgtta tggttaattt taatgtttca gtagatgtca gctttcatat ggcttcctga
721 cgcctagaat tgaggcagat tgacctggtc taaacccagc ctgccttaac gtcttgtgtc
781 tgggactcct tttgcttagg caagagcata aaaagaatag ctcac
//