LOCUS BG069384 713 bp mRNA linear EST 12-MAY-2010 DEFINITION H3076G05-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3076G05 3', mRNA sequence. ACCESSION BG069384 VERSION BG069384.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 713) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG069384.1. Other_ESTs: H3076G05-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3076 row: G column: 05 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..713 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3076G05-3" /db_xref="taxon:10090" /clone="H3076G05" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 190 a 172 c 140 g 211 t ORIGIN 1 agtatttatt tacaagttta ttttaaaagt tttaacagtg atgtctgaca ggaagtcatc 61 agatggccat ccaggtgccc ggcaagagca ctaaaggaga aggaaccccg ggagtccctg 121 gaggctgacg tcagtgcaga agccccgtgt ccccagaggt tgacatctcg gtgcagaagc 181 cctgtgtccc catcagttgc ggaggaagtg ccacacttct ttctcagtct ctctcttaaa 241 ctgacaaata acgtttataa gagacattcc gtaccctcta cattgctttc cttattcatc 301 gaaggtgttg gaaccgattc aaccaatgat cttgcttcct ccatagtgca gtggaaaggg 361 ctatcgcaga tgtgcacatt tacacaactg atggctccct tgtcacactt cttcataacc 421 atctctttcc aacgttcatg tgcagagtta ataacgtcga gggcgaaagc cttgtttttg 481 aattctccat taaaagcaaa cttgttttct ggttttccat ctggtacctt atagagtcta 541 aaccaattga gagtggcttc caggtaacct ggtttgaact tcttaacatc atcaatatca 601 tgaaattttt cagcctcagg atcattcaca ttgatagcaa ttattttcca atctgtttca 661 ctctgatcaa taagtgccaa agtcccaagg atcttcacat ggaccacgtc tcc //