LOCUS BG069364 799 bp mRNA linear EST 12-MAY-2010 DEFINITION H3076E08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3076E08 3', mRNA sequence. ACCESSION BG069364 VERSION BG069364.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 799) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG069364.1. Other_ESTs: H3076E08-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3076 row: E column: 08 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..799 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3076E08-3" /db_xref="taxon:10090" /clone="H3076E08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 248 a 197 c 183 g 171 t ORIGIN 1 agcaaatcac attagaaaga ctgagttagg ttatagaacc agaaatgcaa ctcaagaact 61 ctaggatctc aactgttacc cagctggcat gtacccttgt acatctgggt ctgatggcta 121 gtacctggag tgggacaaga aaggtcaggc tgaataacca atgtggttgg tagtgcacac 181 ccgttgtaca agcactcagg agactgaagc agcactgcca gtttgaagcc gccatggact 241 agtacacaat gagacgatca ctcaaaactg aaaacaaaaa taagtaacac ccagcagcag 301 caaagagtaa acacctgact aaaagaagct taaactgttt tacagataat ttagaccaga 361 gcagcattca caaaatagct gagctgtttc cacctcaaca gggaaatacg aagccaaaga 421 caacccacga tccagagtgg caccactgaa gtcctaactc ctcaactact cccctttacc 481 cagaggacac aatcagtggc tctaagatgc aaacgcctgg cttgttgtaa caaaggaagg 541 acaggaaaag ccttacctgg tggtaaaagt catcatcatc aaatatctcc tcatccaagt 601 ccctcaggtg ggcattggca gggccctgag gaagcgtctc tggttctcct ggcaaggtct 661 ctgcaacagg ctcagggact ggctccggtt tgccaagaac tcggtaggca gagcgcttgg 721 tctgtgtcct ccgaagcaat ctttctttgt ccatcatgat atgatcgatc tgagtcaaga 781 tcgagcgttc gaaggcacc //