LOCUS BG069352 765 bp mRNA linear EST 12-MAY-2010 DEFINITION H3076D08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3076D08 3', mRNA sequence. ACCESSION BG069352 VERSION BG069352.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 765) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG069352.1. Other_ESTs: H3076D08-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3076 row: D column: 08 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..765 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3076D08-3" /db_xref="taxon:10090" /clone="H3076D08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 159 a 197 c 185 g 224 t ORIGIN 1 gtgcttttag ggttatttaa tatttatttt aaaagacagc caatttattt atttattttc 61 aactagagaa tgggtctcta ggtctctagg aattggctgt ggggccctca ctgcacatct 121 tgttccttag cactgtttcc agaatccttc ctcacgtctg gaagaggtgg aggaaaagat 181 taggggtgga tctccaggcc ttcaaagaca tctcctgttc tgaaggccag gcccactgtg 241 gctggggtct ctggccttgc tacctgaaaa gtgaagccac attccctcag agttttcatc 301 tcatcaagga gctggttgtc cttgaacagc agctgctcct caggaggcct cttcatgatg 361 ccctcaatga tgcgcttgag ctcccacacc gtgcttgact ccttggcatc agtgaagatg 421 gtggtcttct tgtgtcggat cacaagaaac acaaacaacc ctgtgtctgc ttgtgactcc 481 atgcacctca ctggaccaga tctgatggct tctcaggttg tcctcagcag gagtgatctc 541 ctagtgtccc tagggccccg actgctcttc cagaggtcct gagttcaatt cccagcaagc 601 acatagtggc tcacaaccat ctgtaatggg gtctgatgcc ttcttcttgt gtgtcagatc 661 tgatggcttc tcaggttgtc ctcagcagga gtgatctcct agtgtcccta gggccccaga 721 tctgatggct tctcaggttg tcctcagcag gagtgatctc ctagt //