LOCUS BG069351 811 bp mRNA linear EST 12-MAY-2010
DEFINITION H3076D07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3076D07 3', mRNA sequence.
ACCESSION BG069351
VERSION BG069351.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 811)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG069351.1.
Other_ESTs: H3076D07-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3076 row: D column: 07
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..811
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3076D07-3"
/db_xref="taxon:10090"
/clone="H3076D07"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 215 a 195 c 197 g 204 t
ORIGIN
1 aaaggcttgg gtataaaaat ttattatacg taaaagaata ttaccactaa taaatgcaga
61 caagcaagga cacaacggga ctggctggaa gatgaattct ttggaaagtg aacaggttta
121 agtggctggg agcctcgtgc caaacaccac agggcatgag ggcaacaagt gactgccgtg
181 tgaatccaca atacctctcc tattggagca agggcctcaa ggatggcttc ccaaggtggc
241 ccaggctgat ctggagtcgg ggcaacacca ctgtaagcaa gacatttaat tggaaaaatt
301 ccacaaacta tacatgaccc ttgagccaca caattcaaat ataaaacatt aagttagagg
361 cgtgggaaaa gaaggcggca tgggtccgaa gctccttcaa tcctggtgga tccgtgggga
421 aaggacgggg atggtgaacc aagcccctcc tctcaatgta caaaggatta ctctgattga
481 tattaaattg tattgacaac aaaatagact aaaaagcaat cgctactctc tggtggggtg
541 gaggtggaga gagaatgagt ccgtgaagca ggaggagcag cggggaggct ctggtcctgc
601 tgctcactcc cgctgctcca tcttgacttt cggtggccgc aggaaggtcc gattcttctt
661 ctccttcttc ccctttgtat ttgcctggaa atttcgccaa ctgtccactc gaccatcccg
721 actttcctca aagtttttct gccactctct ttctcgcttg gctttttcct gggcttcgat
781 ctcctcttct ctctgtcgct tcctctcatg c
//