LOCUS BG069341 794 bp mRNA linear EST 12-MAY-2010 DEFINITION H3076C08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3076C08 3', mRNA sequence. ACCESSION BG069341 VERSION BG069341.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 794) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG069341.1. Other_ESTs: H3076C08-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3076 row: C column: 08 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..794 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3076C08-3" /db_xref="taxon:10090" /clone="H3076C08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 193 a 232 c 205 g 164 t ORIGIN 1 gtttagtcac tttagaggca gaaggaggca ataccatcca tcaggtatca tcagaacatg 61 ttggacagag acatcagtag ctctgggaag cagagaggat gggaacttaa acaaagcagg 121 aaacccaaaa gggccagagc cagatgcccg gggctgctcc cagaactaac cctgccaagg 181 cagcttcatc ccctagtccc ttgtcctggc agtccccaag ttctgtcctt tcttacattg 241 tttcagtctt cattattatc gcgcagtctt ctggcgtcac ctcctcttgc tccacaaaga 301 gttctttaca ttctcgagca ccttgcggcg tggcaggctc aggatagatt ccacaagctt 361 ttgcctccag ctcctgctcc aagctccggg ctttctgcag ctccctatac agcacgcaag 421 catcactgcg ggatacatac tcccatccgc tctcccgaac gtggaagagg tccactgagc 481 cccctgaata agcatcacgg tgggtggcat gggccacagc acagcgggcc agggtgtagg 541 cttcctggat ggtcatgtca tagtggtagc cacgatcaag cacaccatag gcgtagggag 601 acccagagcc tactgagaag atatctccct gcagacaagt gccatcgctg tagacataga 661 agagagcagg gccagagtgg tcccagccac agagggcagt ggcacacaca gatccagacc 721 gcggtacagg acatcatagc tgccaagagt ttggccgtgc tgccacactg ggcagctggc 781 cttcctcagt tccg //