LOCUS BG069168 616 bp mRNA linear EST 12-MAY-2010 DEFINITION H3073E02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3073E02 3', mRNA sequence. ACCESSION BG069168 VERSION BG069168.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 616) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG069168.1. Other_ESTs: H3073E02-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3073 row: E column: 02 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..616 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3073E02-3" /db_xref="taxon:10090" /clone="H3073E02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 166 a 141 c 156 g 153 t ORIGIN 1 gaacatatgc acaaatgctt tattaataca acatacatac agaaactaca aagaagtaag 61 aagtttaaat atctgcatcg taacaaacag gcggcaattc aacatccaga gtggagaggg 121 cactgggagg agagtacagc ggatcagatt cccatgatag aaagggtgtc ttcagagggc 181 taggggggcc cagatgcagc agcttctcca gccctctctc ttcattcagg gtcatgagag 241 gcacgccatt caaggggaga agtgagatct ggtgctcctc aggcaggtca aaactctcaa 301 agtctagagg attgaaaggg aagaactttt ctatttctgg gtaggcatca tcaggagcag 361 gaacagaact ttgtgtctta gtagacttct cggtgatctt tttcccagtc aaggtcggct 421 gttttggttg gaggggtttg ccagtcttca taggcttttc ggcaactctg ttgactgtcc 481 ccaaagcctt tctgctggct ttaggcacgg ctggagcatt gaacactttg ccgactcgag 541 gcgttgaaac ctgcaatttc ccatctaagg ccttgacacc agtgcccagc ttcaacccat 601 ccttagatgc caaacg //