LOCUS BG068957 636 bp mRNA linear EST 12-MAY-2010
DEFINITION H3071B07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3071B07 3', mRNA sequence.
ACCESSION BG068957
VERSION BG068957.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 636)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG068957.1.
Other_ESTs: H3071B07-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3071 row: B column: 07
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..636
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3071B07-3"
/db_xref="taxon:10090"
/clone="H3071B07"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 210 a 122 c 101 g 203 t
ORIGIN
1 aatttagata tgtcacttta tcaaagctag cacaagttca tttataaagg tatcaaaatc
61 acatcttttt acattgaaat cgccacactc agagtactga tttaaacccc tctattcaaa
121 aataactgca tgtacactat taacacctta gcctttaata taacatagat gaatggactg
181 aagaagattc agctttggct aagaccggtc ttgaaaaaac acttaagacc acagctgttc
241 caattttctt ttcgccttgc agaagaatca ttcaaactag ccaaagtttc gttctctgac
301 acatgtattt tgaatttgaa aagcttaatc ttaaactcaa gctctgtgag ccaatttcag
361 ccacaacaaa ttttggcatt gggttctaag ttctgtataa tagcataggg cgcctggctg
421 ccttaactaa ggagggatga gaagggggag catataactt aacgctttga agagtaagta
481 tgtcagaggc tgccattctt acattgaata ttccagcatt atttatttga tcaaagtaaa
541 atacactttc ccattgctat aaactacagt tgttcacaca cccacatttt ttttaaacga
601 tgtgtcaaca attttgtatt gtacataaaa tgtttg
//