LOCUS BG068807 709 bp mRNA linear EST 12-MAY-2010 DEFINITION H3069E06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3069E06 3', mRNA sequence. ACCESSION BG068807 VERSION BG068807.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 709) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG068807.1. Other_ESTs: H3069E06-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3069 row: E column: 06 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..709 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3069E06-3" /db_xref="taxon:10090" /clone="H3069E06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 214 a 181 c 127 g 187 t ORIGIN 1 cttcagcata acattttatt aattatctgg gaatttcaca tcatgtactc caatcacact 61 cagttcccag tccttccatg tcagccccca cccttgttac ttgcccccca aaagaggaaa 121 attaaaacta agaattaaaa aaaattaaaa aaattaaaat acacaagtcc aatttatgtt 181 acctatatac tcactgaagc atggtcagat tctcagtggc ctggccctta aatagaagtg 241 agtcttcccc ctcctgcatc tgaagccatt aattgcgtag agctacactt caacatctac 301 atcacacttt caaagagttc tcttcagtgg attcctgttc tagaccatta ctttggagac 361 aggggtaggg gattgtcaca aaaagccttc catgtccctt cgtctcagtt gtacacctgc 421 agtcatcaat atcccagtgt aactagtccc cttgttcttc atagttagtg gaaacatgga 481 tcatgggcct ctcaaggttt ctgataacat cccagaccat gaacatggcc ctcaggtgca 541 ggaggaccac agatccagac aaagaccaga tcacagccat aaaaatagcc tcaggtggca 601 gcaaaggcca ctcagattag tatggccttt ggtggcagca ctatccataa acatcaacgt 661 agcttcaggc agcagcacag actgtgaaca tctcatggtc ttcagtggt //