LOCUS BG068797 728 bp mRNA linear EST 12-MAY-2010 DEFINITION H3069D08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3069D08 3', mRNA sequence. ACCESSION BG068797 VERSION BG068797.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 728) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG068797.1. Other_ESTs: H3069D08-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3069 row: D column: 08 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..728 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3069D08-3" /db_xref="taxon:10090" /clone="H3069D08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 192 a 176 c 168 g 192 t ORIGIN 1 ctttttttct cccttctttt caatttaatc tttgagcccc cacagggttc tttatgttag 61 cttcatgctc agtaaaatgg ccaagtttat ataataagtt ctagatgacc aggatagcta 121 ctgcccctca aaaccagaaa gccaggcagg agaggcaacc tgaagccagc tgggtggttt 181 aactccagaa ctttccaggc aaatacagga aagctgctag tcagcctagg ctacataata 241 taacctgtat caaaggtact tgctgccaag gctgacagcc tgagttcaat cccggggacc 301 tacactaaag atcatggact ctaagttgtc tctgacctct gcacatgtgt gtataatgac 361 aatctgccac ctgaagttac tgagggggtt tgtcaaccct cctactcccc aggccatctt 421 aaatggccta tacactttta tttgggatat tcaaagccaa ggtataggaa ggagatcaag 481 tgaaagggaa agaatgggaa gtggcccaag gcaacagttc caagatggcc aaccttgaag 541 atgcaggcat gtccgctggc tgtgtagcag gtctccccac agattgtgct gtcagcgata 601 cgatgtcttg caacaattca tggaggttgg ggggagattt gctgcctgcc tctctcccat 661 ctcccattca tattagtctc ctcacaaggc ttggtttggg tggcccatga aatgccagat 721 gccatgtc //