LOCUS BG068724 856 bp mRNA linear EST 12-MAY-2010 DEFINITION H3068F07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3068F07 3', mRNA sequence. ACCESSION BG068724 VERSION BG068724.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 856) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG068724.1. Other_ESTs: H3068F07-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3068 row: F column: 07 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..856 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3068F07-3" /db_xref="taxon:10090" /clone="H3068F07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 223 a 209 c 189 g 235 t ORIGIN 1 ttttttattt tcaaagcctt tgtgaatcaa agactagttt ggtgctgact gcctgggaga 61 gtggttagta gggcctcaga ttattaacat tttaaaaata agtattctgc ggcctgggga 121 tgcagctcag ttggtagagt gcttgcctaa catgcatgac accataagtc ccatcccctg 181 tcccccacac atataaacca agcaaagcaa cacacaccca aagtcccagc acgtggacag 241 ttgacactag tctggattat atgagattcc atctcaaaaa gagaaggaaa agaacccttt 301 tctcaggagc ccttctcact gtgtggaggc agagccccat ggagtggagt ttggtagctg 361 ggtgtgtatt aagttctgtc agaagcccac cgtctgttat gaacctctgg cctaagttgg 421 ttttatatcc cagtccagca gtccagcctg ggacatatgc tcttttccat caatagtttt 481 ttattcttgt ctgtcttcta gactcatagc tgcgcagaat gtgccgtgtg tatctaccag 541 aattgcactg ctgtgaggtc agacctatgg agtcagttgt tgtcttacaa agacagttcc 601 caggagtttc ccttgccatc aggagcagga aactagatga taccatctga tgagcccagg 661 ccagctttat gtctcaccat tccaggaaca gctcacttta ggacacctag gaatgaacag 721 atctcattgt ttgatatata ctttctaaac tctccacttg ggacagatga acaagataga 781 ggtctagggc cagtagctga gcctcacccc caagccctta aactctgccc tcttgctcag 841 cctcccagag ctagga //