LOCUS BG068704 558 bp mRNA linear EST 12-MAY-2010
DEFINITION H3068D11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3068D11 3', mRNA sequence.
ACCESSION BG068704
VERSION BG068704.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 558)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG068704.1.
Other_ESTs: H3068D11-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3068 row: D column: 11
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..558
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3068D11-3"
/db_xref="taxon:10090"
/clone="H3068D11"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 137 a 138 c 117 g 166 t
ORIGIN
1 atatgcccat cagtgcaatc tgctttattt gacataaggc aagtgggaca gtctcttttg
61 aatggatttt tgtcttttgg aagagccagg taagagtttc attcttaaaa ccacgttttc
121 tttaatgccc aaacatgtca atttcgtctt cctttctggt cactccttgg cagtcagcga
181 gtagtcaaag attaatgtcc gagcaggccc gtacctttaa agccctcttc cggcatacaa
241 acttgcatga catcatccat tgcagcgtag tctgaattag gttcatcagc ctttaacaaa
301 atgacagact caggaatgac gccgagggta ccaagggtgg cacagtcgtc gtttaaaatc
361 tttccatcaa ttgacaaatt ctggtcaaat ggagcaactg aaaatgcgtg catgatctga
421 atcttcaatt cttttaacgt ctggttggca gacaccaaca gtgccttctc tccccgaacc
481 ttcctgtgcc tcgtgctcct ccggatgacc tgcttctggt aggccacaca gccctgtagg
541 gatgtcttct gtcgcttg
//