LOCUS BG068662 811 bp mRNA linear EST 12-MAY-2010 DEFINITION H3068A04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3068A04 3', mRNA sequence. ACCESSION BG068662 VERSION BG068662.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 811) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG068662.1. Other_ESTs: H3068A04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3068 row: A column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..811 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3068A04-3" /db_xref="taxon:10090" /clone="H3068A04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 248 a 177 c 190 g 196 t ORIGIN 1 ctgaagctct agttgtcatg gactggaagg cagacttgca tctcgaaggt aaaattaggg 61 aggggttgac tggaaccata aagggaataa aacaagaaaa tgagaatggt gggataggga 121 gatgttcagt ggttaagggt gcttgctgca ttcccagaag accagagttc agttcccagc 181 atccatttta ggagacttca caaagccatg taatttgagc tccaggggat tccacacagt 241 cacctgcctc agagggctcc tgtatgcata tagtacaagg cacacacgta cattaaggta 301 aaataaacct tataaagtga atgataaaga gatggcctag ggtctctaga ggaagaggtt 361 agtgaagaca tcgatccacc cacaaatgct cttcagagtg agcagagatt acataggggg 421 aatgacacgg tcaggggcac tgactgaaaa taggtcacgt catattgctc ccacatactc 481 caaagactcc taccagtact acaaatactg tgcacacacc cagacacaca ccctgaggca 541 gaggaatgaa catgtctgct taggacacac gccccagctt cctaccaaat gtaaagattc 601 ccctctttct cttcttccag ccatattttt cattcatttt tctgaaggaa aatttagata 661 tttacttgtg tgtgtgtgtt taagtctgtg tacaacacac atgcatatgg tacctgcaga 721 agtctgaaga ggccatcaga cctcctagaa caggagttaa aggccatggt gagtcatccc 781 gtgggtgctg gaaattgaac cacatcctct g //