LOCUS BG068541 600 bp mRNA linear EST 26-JAN-2001 DEFINITION H3066F09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3066F09 3', mRNA sequence. ACCESSION BG068541 VERSION BG068541.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 600) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3066F09-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3066 row: F column: 09 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..600 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3066F09-3" /db_xref="taxon:10090" /clone="H3066F09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 179 a 153 c 123 g 145 t ORIGIN 1 aaaaacttac tttaattacg gttcttaatt aaacctctct tctagcccac cacccagagg 61 tagtgggaaa agaaggttaa aaggaaaggg gtctgtagac ctgcttagaa gtagtccttt 121 gggggcaatt ccaatcttcc ttgtcaggat atcagcagtt aagtccaaca gcaaacacca 181 agcacaaatc agtagcagtg gctccatcca gctgaaaccc caaagatttg ccaaatcagc 241 acgagtcagc agaatcagcc cgagtcagcc ggaacaccac gagaagttct tcgttgcctt 301 tctctctatg aagtgaagac caaagaatat cagcggagca ctgcacagct tagcgacacc 361 agggagactg tctgtggagt tatatttata ttctgcttaa acatcatgcc ttctctcaag 421 cgccagctcc agcaaagcct atgccctccc tcgcatgtgt ctgcttcagc aaaacatcct 481 ctcgcaagac agctttcgga aaagcatcac gtgacacaac tgagtttcca aaggaaccag 541 aaatttccac ttcaggttta tttggcttac agttccacat tgatgttcag gatagtcgag //