LOCUS BG068528 601 bp mRNA linear EST 12-MAY-2010 DEFINITION H3066E08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3066E08 3', mRNA sequence. ACCESSION BG068528 VERSION BG068528.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 601) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG068528.1. Other_ESTs: H3066E08-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3066 row: E column: 08 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..601 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3066E08-3" /db_xref="taxon:10090" /clone="H3066E08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 160 a 137 c 137 g 167 t ORIGIN 1 cttataaaca aaattcctca ttttctaaat gcatggtaga gacctttgca actatcttct 61 gcagctggct ttaaacggga gctgtgaagt gctaggcctg tgactctctc catatctggt 121 gtccatcctt cctagagagg ggttcagcat cttgaagcgc tttgttgaac tctgtcttta 181 tggctactgt gcttccttcc ctgtgctctg cgaagcttct cacctcagtg catgtagctc 241 tgcagacaag ggatgaagag atagggcgct tggcagatga agtcccagca cagagttcaa 301 gccttgcttg ggacccaggc aagactgaaa ttgaaggctt gcccttctac ctcctagtta 361 ggacctgcag gcaagtctct tcactctgcc ctccccagtt aaaagactgt ggctctaaat 421 gtaagtatga agatgaagtg agatgaagta accagttctt gacataactg ctgccatgat 481 tgtttcccag aactgaagat gaatgtgtta tcaatttgac aagatctaga gtcacttagg 541 agacaaacat ttctagaagc atttaactaa tgagaaaaga cctaccctga caatgggcag 601 g //