LOCUS BG068443 662 bp mRNA linear EST 26-JAN-2001 DEFINITION H3065E11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3065E11 3', mRNA sequence. ACCESSION BG068443 VERSION BG068443.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 662) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3065E11-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3065 row: E column: 11 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..662 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3065E11-3" /db_xref="taxon:10090" /clone="H3065E11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 189 a 129 c 131 g 213 t ORIGIN 1 ggtcagcttc atgcacaaaa ttttttgcca gctttctttt tgatatactg ctcttatttg 61 tgaagtgtct ttgttgcttc ctcacttctg tatctgtgtg gtagttttct cctttcattt 121 cctctcagga tttcactgtt gatattctag ccttatcagg tatctccttg tgtttcttcc 181 tcaatgtttt tgtttagtta gaggacttta ttttagcaat cacagcacat gtggcatgca 241 aaataatgtt ccgtaataag ctcacatcct aatctggaga gctagtaata tgttatattc 301 tgtgctcaag aggcattata gctttgcaga tgaaattatg cctgccagtc agctgatctt 361 caaaatagct tatcctgaat gatgcaggtg ggccctaacg taatcacaag ggcccttcac 421 agcagaaaca ggaaatagaa agagactcag tgtcagagtg aagcaatgga agaaaaatta 481 cacagctgtg tttggcttta ggaaagggac catgagtcaa aatatgtagt agaaacttac 541 cagccaagac cgtacgtgtg cctagaaact tgaaaaggtg aggaagtgca ttatccttta 601 aagatattac attctcatgt accctctaaa gcataccatt cagaccagag agaccaattt 661 ag //