LOCUS BG068405 704 bp mRNA linear EST 26-JAN-2001 DEFINITION H3065B04-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3065B04 3', mRNA sequence. ACCESSION BG068405 VERSION BG068405.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 704) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3065B04-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3065 row: B column: 04 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..704 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3065B04-3" /db_xref="taxon:10090" /clone="H3065B04" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 213 a 154 c 165 g 172 t ORIGIN 1 ggcaaaacaa ctaaaaccat ctccttaaac tttctctcta aaattttttg aatcaaacca 61 ggactactat aaagtcccca gttcatctaa acagcatttt ttcatgaaag ttcatcttca 121 tgttgacctg aagggaaatt ttcccaatag ggtgggctga tgcccaggca tcattaggct 181 ctgtaacagt ggcaggaaag gcacatcagc agcgagaggc aatcctggga tgaagtctct 241 ggggctgtgc ctctccagag tgcacccatc acagccatgc aaaatggtgg gccatctcca 301 ggaaactcac gtgcttgccc tagcctttcc tagatggctt ctgccactca ggactgcttg 361 ttcatgtctg gttctgccca cagtgggttg ggctgttgca catcaatcat caatcaagaa 421 aatgcccctt accggtagag aggttttaat tcagcaacat agctactctg actggagtac 481 agtcatgccc ttactatacc tctttagtcc taaacagtga aaagttagct tgtagaagga 541 agcacctata tttgaagttg agattgagag gcaaacaaag tgatgaaaca gagaaagatt 601 tgacaggatg gaatatgccc aactctcagg aaaacagagc gggggaaggg ctacttaaga 661 gacaggcagt agagaaagga aaagaggaga ggacagtttt actg //