LOCUS BG068319 532 bp mRNA linear EST 12-MAY-2010 DEFINITION H3064B09-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3064B09 3', mRNA sequence. ACCESSION BG068319 VERSION BG068319.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 532) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG068319.1. Other_ESTs: H3064B09-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3064 row: B column: 09 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..532 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3064B09-3" /db_xref="taxon:10090" /clone="H3064B09" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 184 a 99 c 89 g 160 t ORIGIN 1 ccaatttaat ataaaacttt aatgcagaat taggacaagc aaaaaggcat tttgcagttt 61 agagatagaa tttcggaata tacaacagaa ggtctcacaa cctaaattcc agcagctttt 121 taaaaaaaaa aaatctaaga acttaagact gaatctactt agaatcagtc ttaattgtgt 181 aaaaatagaa atgaaaataa gctgttgtat gaataggctt ttattttttt ttcctgagtg 241 cctttgagcc cccaatatct cagtagtgca ttctttatga agactcattc tttatgaaga 301 cataactctg gagaattcta gagcctcctg gcctccgtac aaattaccca gttccaccca 361 ccaccccata ttgaagagtg aacataataa atttgcatga aaatgaagga ctagcagttg 421 ctgccttgag taagactgct gatggtatta tttcatatgc acaagtgcat atatctaagc 481 agattggctg aatactccta aaccccagca gaattctgtc ctgtggttta at //