LOCUS BG068286 663 bp mRNA linear EST 12-MAY-2010 DEFINITION H3063G08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3063G08 3', mRNA sequence. ACCESSION BG068286 VERSION BG068286.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 663) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG068286.1. Other_ESTs: H3063G08-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3063 row: G column: 08 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..663 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3063G08-3" /db_xref="taxon:10090" /clone="H3063G08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 231 a 130 c 133 g 169 t ORIGIN 1 gaccatattt tattgataga attgtgaaat agttatttta aaataaatca gacacaaaac 61 aaatcagatc aacaatcaag aaacaaagta aaataataaa ataaaaacaa tcaaagaaaa 121 acatgccacc atatctacac acaagaagta aacatataag aaataaaact gaaataaaca 181 tgcacaaatg gcaccaagaa agatggaata catccctgga gaaggaaaat taaacatgca 241 gaaagagaag agtttgagtt ttgagagcaa caggatagtg tgcggaccat ttaccttcac 301 tgtcagacat agcatgttgg aagtcatcca tgatgaatgc tatatcacgg tcataacccc 361 ttgtccgtga ctcttctctc atgtgttgct gaacttcagg caatgagtga cttgatccaa 421 attgatccct tgtatctgca gatatgggcg gcaaaggtcc agctgcagcc ctggccattc 481 ccattggctg gccaacagga cttagtggac tttcttcttc tgaattctgg gccacaattg 541 gtattcttcc tctactctgg ctgattggta agctggtggg cttagttctg gcagaagatg 601 aagaatggcc aaaagagaca tccagagctt cagcctcttg agctttcaat cttagtgatg 661 agc //