LOCUS BG068281 762 bp mRNA linear EST 26-JAN-2001
DEFINITION H3063G03-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3063G03 3', mRNA sequence.
ACCESSION BG068281
VERSION BG068281.1
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 762)
AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set
JOURNAL Unpublished
COMMENT Other_ESTs: H3063G03-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3063 row: G column: 03
Seq primer: -21M13 Forward
POLYA=No.
FEATURES Location/Qualifiers
source 1..762
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3063G03-3"
/db_xref="taxon:10090"
/clone="H3063G03"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 144 a 246 c 117 g 249 t
ORIGIN
1 ccttttttcc tttgattagt acgaatgtcc ttccttatct atttttgata actttaagtt
61 ggaagtcgat tttgttcaat attagaatgg ctactccagc ttctttcttg ggaccatttg
121 cttattcaat catgtctcct ggacccctgg ctcctgtcaa agttactgct gccacagtcc
181 ccacaataga ggcacgtggc tagcaatcac atagacaatg tccaaagctt ctggcattct
241 ggatatactc ctccccacag ttacctggca acagcaagat agcagcctac tataagaggg
301 gctgcttggc ccctcctcac tctctttaag ctttcttact ctctagctct ccttctgtct
361 ctctttttct cttcccatct ctctctctct ctctaagctt ttacctctct tactctctag
421 cctttcttct ctctctctct tcccccttct ctcctcctgg ccatggccgg tctctctctg
481 ccttctctct ttccccctgc ctttctacaa taaagctcta aaaccatcca ctgtctctgc
541 tcatcaaggc ccgcagtnnt gaatgatcgg ataggatttc tcctaaagag ccatgcttaa
601 tctcccaaca gaaggccttc ctatgctaca gccatggact gaactaagga ctctagcctg
661 cttggggaca cccagcaccc ccttctncct ataaacncnn ccctgatctc ctgggccaag
721 tgtctccccg ggcctcctat tctgttctca gcttctctgc tg
//