LOCUS BG068248 653 bp mRNA linear EST 26-JAN-2001 DEFINITION H3063D02-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3063D02 3', mRNA sequence. ACCESSION BG068248 VERSION BG068248.1 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 653) AUTHORS Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A., Tanaka,T.S., Carter,M.G. and Ko,M.S.H. TITLE Verification and initial annotation of NIA mouse 15K cDNA clone set JOURNAL Unpublished COMMENT Other_ESTs: H3063D02-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3063 row: D column: 02 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..653 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3063D02-3" /db_xref="taxon:10090" /clone="H3063D02" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 107 a 137 c 155 g 254 t ORIGIN 1 gaggtgctgt ctgcctttga tgtgaaggtg tgtgtcctgg atgcagaagg acggaccgtc 61 tttctgtgcc cattctcttg gtctgtgtct tgttattggg gtgtcgaggc cattgatgct 121 gagaaccatc agagagcagt atttgttgat tcccattatt ttgttgtggt gatgtccccc 181 acccctgatt tggtggtctc tgataattcc ttgtgtttcc ttgggtgtgg ttaccctctt 241 taggttggag ttttccttcc agcaccttct gtgaggctgg atttgtagat ggacactgct 301 gaaatttggt ttttatcatg gaatgtctct tttttccccc ctccatctat tgtgattcta 361 agttttgttg ggtgtagcag tctgggctgg catctgtggt ctcatagtct gtagagcatc 421 tattcagact cttctggctt ttagtctctc ctgggaagtc aggtgttaat atgataaacc 481 tgacttttcc ccttgcagcc ttgaacattc cctttctttt ctgtatatgc agtgttttga 541 ttattatgtg ccaaggggat tttcttttct ggtctaatct agttggtgtt ctgtatgctt 601 cctgttcctc gacaggcatc cctattctag tccctaaatt tctcacttgc aga //