LOCUS       BG068183                 578 bp    mRNA    linear   EST 26-JAN-2001
DEFINITION  H3062F07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
            H3062F07 3', mRNA sequence.
ACCESSION   BG068183
VERSION     BG068183.1
DBLINK      BioSample: SAMN00163148
KEYWORDS    EST.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Kargul,G.J., Dudekula,D.B., Qian,Y., Lim,M.K., Jaradat,S.A.,
            Tanaka,T.S., Carter,M.G. and Ko,M.S.H.
  TITLE     Verification and initial annotation of NIA mouse 15K cDNA clone set
  JOURNAL   Unpublished
COMMENT     Contact: George J. Kargul
            Laboratory of Genetics
            National Institute on Aging/National Institutes of Health
            333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
            Email: cdna@lgsun.grc.nia.nih.gov
            This clone set has been freely distributed to the community. Please
            visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
            Plate: H3062  row: F  column: 07
            Seq primer: -21M13 Forward
            POLYA=Yes.
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6J"
                     /db_xref="niaEST:H3062F07-3"
                     /db_xref="taxon:10090"
                     /clone="H3062F07"
                     /sex="clones arrayed from a variety of cdna libraries"
                     /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
                     /dev_stage="Clones arrayed from a variety of cDNA
                     libraries"
                     /lab_host="DH10B"
                     /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
                     clone is among a rearrayed set of 15,247 clones from 11
                     embryo cDNA libraries (including preimplantation stage
                     embryos from unfertilized egg to blastocyst, embryonic
                     part of E7.5 embryos, extraembryonic part of E7.5 embryos,
                     and E12.5 female mesonephros/gonad) and one newborn ovary
                     cDNA library. Average insert size 1.5 kb. All source
                     libraries are cloned unidirectionally with Oligo(dT)-Not
                     primers. References include: (1) Genome-wide expression
                     profiling of mid-gestation placenta and embryo using a
                     15,000 mouse developmental cDNA microarray, 2000, Proc.
                     Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
                     cDNA analysis reveals phased gene expression patterns
                     during preimplantation mouse develolpment, 2000,
                     Development, 127: 1737-1749; (3) Genome-wide mapping of
                     unselected transcripts from extraembryonic tissue of
                     7.5-day mouse embryos reveals enrichment in the t-complex
                     and under-representation on the X chromosome, 1998, Hum
                     Mol Genet 7: 1967-1978."
BASE COUNT          159 a          145 c          109 g          165 t
ORIGIN      
        1 cactaaggca gaacttacag gaagtgtgag ttatggactg acgtaattat agaacgatca
       61 ccaagcactt gtgtgtctag atactggctt gtgtgtcccc tgcttcatgt gaccacaacc
      121 gatgctggtg tggtaaattg tgcctactga agagaggagg aggacagggc tcagagaagc
      181 caccctgttc acacagcata ggttccctcc agcagacact gtctctctac tttcccttca
      241 tgaacacagt tttaggactt tagaaaccac cttagaatct aagcttgcat ctccagctct
      301 gatcacttga ccacctagct ctcatttaat gtcagatcct atctatcgta ctattaactc
      361 ttatctatct cttcttttgt tggaaatata aaacattggg gtgcctaggc taccaggctg
      421 cttatactaa cctagactgg gccccctgtg ctcatactgc tattgaagaa atctcagaaa
      481 acaagaaaat tccatattta tgtccaggag ttccatttgc cacccaccca aggaacccca
      541 tgttttctaa acaccttttc taaccgatag attacatg
//