LOCUS BG068101 771 bp mRNA linear EST 12-MAY-2010 DEFINITION H3061G06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3061G06 3', mRNA sequence. ACCESSION BG068101 VERSION BG068101.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 771) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG068101.1. Other_ESTs: H3061G06-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3061 row: G column: 06 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..771 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3061G06-3" /db_xref="taxon:10090" /clone="H3061G06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 181 a 202 c 138 g 250 t ORIGIN 1 attttcttta tattgattta aaaaaatgtc tatcttacat ccctaccagt ttctcctctc 61 tcctccccag tactggtctc tccacctgcc cccctctgtc cacagtctcc tattgattct 121 ttccctccct tcctctttac ctaccccatg cacgactctg tttctcttta gaaaagggaa 181 ggcctctcta gatgcatatc aacctgcctt ggcatatcaa gttgtagtac gactaggcac 241 ctcatgttct actaagacta gacacggcaa cccagttaga gaaaagggtc caaaggtcag 301 agacaactcc ttcatccact gttagaatcc cacaagtaga tcaatgacac aaccttaata 361 tatgtatgac gggcctaggt tagtcccatg cagccttcat ggttgtctct tcagtctctg 421 tgagccccta tgagcccaga gaggttgatt ctctgtttgt ttgtttgttt gttttttgtt 481 ttttcatcca taacctctct ggcttctaca atccttctct ctcttctgtg gggttccgca 541 agctcagcct aatgtgtggc tgtgggtctg tgcgtgtgac aatggaacat tattttagga 601 gaaaacctga tgacttatga caaagcttgc actcacatga ccactggggg gacatttgga 661 tttagatcat ttccatcgca ccaagaaaat cccctcaggc tctcacatga tcagtcttct 721 cagcttttac atggtgaaag acactggtgt actttgaaaa catcattttt t //