LOCUS BG068045 691 bp mRNA linear EST 12-MAY-2010
DEFINITION H3061B06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3061B06 3', mRNA sequence.
ACCESSION BG068045
VERSION BG068045.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 691)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG068045.1.
Other_ESTs: H3061B06-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3061 row: B column: 06
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..691
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3061B06-3"
/db_xref="taxon:10090"
/clone="H3061B06"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 232 a 117 c 116 g 226 t
ORIGIN
1 aatttataaa gtttatttat tttttaaaaa gtactctgag aaaacacaca gtatagtcac
61 ttcagaaaag ctatttgggg aagactcatg tgcatcaagt ggagtgggct tctaaaaaat
121 atttgtaaat tttccaaaaa tttaaatgtt aaaaatagtc ctctccttat caacttcatg
181 tttaaggaaa tatttgacag acaagtcaac aagagacaac aaaagtttac ctggatttta
241 aaacaatgaa ttctagattg ataaaaagtt cctggtttgt accgaaaggc caaatgattg
301 gccttcattg atccatttct atatagttat aaaaatacag tatcaaatct ttttctattg
361 cccctaaaat taggggaaac attttcagct gtgagtgtgt ggatgcaggt gtgtagatcc
421 ccaagtgtac aaccacacct cactttacta aggaaattat tttcttcaca ctgtaaacat
481 ctctcttcta catctggaca tttcagaatc gtctttggta ttctagttgc tgtgctttca
541 gacagaaacc ccagggtttc tgcagcggtt ctacatagac atcttaagat ataaataagg
601 aaatgcactt ggaataagtt acaattagct gcttttgcat aattttcaac aactacagtg
661 tatgcctagt cacagtttta tgaggaagag t
//