LOCUS BG068005 683 bp mRNA linear EST 12-MAY-2010 DEFINITION H3060F11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3060F11 3', mRNA sequence. ACCESSION BG068005 VERSION BG068005.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 683) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG068005.1. Other_ESTs: H3060F11-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3060 row: F column: 11 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..683 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3060F11-3" /db_xref="taxon:10090" /clone="H3060F11" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 209 a 121 c 125 g 228 t ORIGIN 1 cacatgtgac tagtctagca gtcatctaga ctctgtcttg catttctgga tgcccataaa 61 atatcggatg tgattttact cttaacccag ttatttgggg attacagttt ctaaaattta 121 tattttagga agatggtgat gctggtacta gtggtgatga aaatgctgca attaaaaaaa 181 aaaacaagtt attatctatg cacataagat ctatttgtat aggtatttaa gccaaagaag 241 aaatggattc taagcaggat atgtattatc atgtatacat ttcttagaat tcagttcaaa 301 gtttggctgg agcagatcat ttctgtcagt aaataaatgt gttcttcttt ttcagcttac 361 agactggaac tagaagtgtt agccaccatc atcataggag aataggtctc tgaattcatg 421 cctcctccct tcaaatgaca gttggatgaa taccagtaga atggcctgac tctccctatt 481 ggttcatatc tttctctcta ctcccagggg aatcaggata ctttggtgtg taaaagaaac 541 agaagggtaa ttctgtctct ggccatacat tccattatga tatagttctg aatgtttgca 601 tctatttgtt caatcattca ttcattcatt caataaactg accttgaaca ccaattctgc 661 caaacaccat ggtaaatgta gag //