LOCUS BG067994 697 bp mRNA linear EST 12-MAY-2010
DEFINITION H3060E11-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone
H3060E11 3', mRNA sequence.
ACCESSION BG067994
VERSION BG067994.2
DBLINK BioSample: SAMN00163148
KEYWORDS EST.
SOURCE Mus musculus (house mouse)
ORGANISM Mus musculus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE 1 (bases 1 to 697)
AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X.,
Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H.,
Wood,W.H. III, Becker,K.G. and Ko,M.S.H.
TITLE Genome-wide expression profiling of mid-gestation placenta and
embryo using a 15,000 mouse developmental cDNA microarray
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000)
PUBMED 10922068
COMMENT On Jan 26, 2001 this sequence version replaced BG067994.1.
Other_ESTs: H3060E11-5
Contact: George J. Kargul
Laboratory of Genetics
National Institute on Aging/National Institutes of Health
333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA
Email: cdna@lgsun.grc.nia.nih.gov
This clone set has been freely distributed to the community. Please
visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details.
Plate: H3060 row: E column: 11
Seq primer: -21M13 Forward
POLYA=Yes.
FEATURES Location/Qualifiers
source 1..697
/organism="Mus musculus"
/mol_type="mRNA"
/strain="C57BL/6J"
/db_xref="niaEST:H3060E11-3"
/db_xref="taxon:10090"
/clone="H3060E11"
/sex="clones arrayed from a variety of cdna libraries"
/clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set"
/dev_stage="Clones arrayed from a variety of cDNA
libraries"
/lab_host="DH10B"
/note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This
clone is among a rearrayed set of 15,247 clones from 11
embryo cDNA libraries (including preimplantation stage
embryos from unfertilized egg to blastocyst, embryonic
part of E7.5 embryos, extraembryonic part of E7.5 embryos,
and E12.5 female mesonephros/gonad) and one newborn ovary
cDNA library. Average insert size 1.5 kb. All source
libraries are cloned unidirectionally with Oligo(dT)-Not
primers. References include: (1) Genome-wide expression
profiling of mid-gestation placenta and embryo using a
15,000 mouse developmental cDNA microarray, 2000, Proc.
Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale
cDNA analysis reveals phased gene expression patterns
during preimplantation mouse develolpment, 2000,
Development, 127: 1737-1749; (3) Genome-wide mapping of
unselected transcripts from extraembryonic tissue of
7.5-day mouse embryos reveals enrichment in the t-complex
and under-representation on the X chromosome, 1998, Hum
Mol Genet 7: 1967-1978."
BASE COUNT 199 a 135 c 143 g 220 t
ORIGIN
1 acatttgaac agtttatttt actattgtag tctactctgc actgttaggt tctctggccc
61 catacagtat acttttggaa cgtacaaact tggtgtagtt tacttacatt ttaacaatgc
121 tcatgccatg attataaaag attagcacca gtctctactg ttctcaactt ccacaccact
181 gtggcttcct gtgtagctag gatggaatta ttcaaactta tttctttatt gaagccacac
241 caaaagatgc aaaccaggag gcatttcatg atcctggcca acatatgtac ataaatatgc
301 ttcacaaact gtgtaagatt aaggaaactt acatcaatta atatattacc caattttttc
361 caatgccaaa tgctacctca gcttttgttc tcacctgatg tccacacatc taatttcatt
421 gcctgagagc tgtccattta gaacaacaca gtatgaatac gggttatttc aaaggtgatc
481 tgagacgtgc agttatctct ggagctagtg aaggatagaa tgaatgttag gttcttggct
541 gcaaggctcg gtatgttgaa gtgcattgat tggatgcttt gatagagaga gaagggtttg
601 cagtttccac cttcccacaa ggtttagttt gggggacaaa gggtgaggtt acgggacagg
661 tgtgagagca atcctggtta cagtctagtc caaaagt
//