LOCUS BG067917 705 bp mRNA linear EST 12-MAY-2010 DEFINITION H3059F08-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3059F08 3', mRNA sequence. ACCESSION BG067917 VERSION BG067917.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 705) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067917.1. Other_ESTs: H3059F08-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3059 row: F column: 08 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..705 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3059F08-3" /db_xref="taxon:10090" /clone="H3059F08" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 201 a 172 c 168 g 164 t ORIGIN 1 aatacataga aagaaatggg ttttaggcaa atgagaagac ttaaaagata cattaacaca 61 cagaaagcca gagagaccag aaatttaaat ccaaatgtgc tgggggtggg gtgggagaaa 121 gaccatttct agcagaactt atgattgaca ggaggagtgg tgtacagatg tatgaaatgg 181 atcttaggac aggagagact ggtccacgtt ctggaaagac ctcttcattg atgaccatca 241 ccatccaatg atcacagatg caggcattca cccatgtgat gtgaaccagt tcccactggt 301 ttgtgggctc tgccacctaa aacaagcatg ccaggcaact ctgcttgtcc tagtcttaag 361 aacactgcca gttgctcaca gtagactgcc cttgtgtatt ggaccaaccc tgagcccaga 421 catcgtgggc tatgtcctgt aagcactgaa gaaaccaatg ctagccatca ggaccggaag 481 acgtgcactg ctctgatcac atactgccgg cagataggac attcgttcat gcgtttgcca 541 cacttggtac aggtcaccat gtggccacac tccagcagaa cacagtcaat gggtgagtcc 601 atgcaaatct tacacaggtt ctcctccagg ccagaaggca ctgctccccc gttttggtct 661 tcattaccag acaccaggtg ctggagtcct ttctgatcct tgtac //