LOCUS BG067915 787 bp mRNA linear EST 12-MAY-2010 DEFINITION H3059F06-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3059F06 3', mRNA sequence. ACCESSION BG067915 VERSION BG067915.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 787) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067915.1. Other_ESTs: H3059F06-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3059 row: F column: 06 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..787 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3059F06-3" /db_xref="taxon:10090" /clone="H3059F06" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 126 a 215 c 203 g 243 t ORIGIN 1 gaatacaaag tctttattga taaattatga ttaaacattg tgacagctta ttagacaata 61 aaagcttcag aatcgccatc tccccagccc tggccctagc tactagcctc agccttcttg 121 ctgggagcct tggaggaggt cttgggaagt aactcaggcc tgagtgttct caggggtctt 181 tgccttcctg accctaggta tgggttgtac tttgtgtcct atgtcctggg aaggagtggg 241 cactttggtc tcctgtactg tctcagcccc ctgaacaact gtcacagtgt gggccatgtc 301 tgccatcttc cttttggcga gggaagcaac gctattctgg accttgctgg ccaagggctt 361 cgatttctca cctttggttt tcccatgggc atttgcttct tgcctgctcc cactgcgttt 421 gaccttctgg cctccattgg cagtcagagg ggcccctgct gctttgtctt gttttagggg 481 agcctttctg acctctttcg gtttcgcctt ggctttctta ggtgccatct ccagtgtggc 541 tgccttgcaa gggtaagccc tcctcttctg ccctttctcc atcgtggcct tgccaacttg 601 gtctttcttc agcaatccag atttcttgga gcctgtctcc ttggcacctg cagctcccct 661 gccggctttg ggggcacagg ctttctttgt cttgggcttt ggaactagtt tgaagctgcc 721 agtggcaccc ttggccttgg agtgagcagg cctggtgagg aggctcgacg aacgcagttt 781 ccagagc //