LOCUS BG067893 810 bp mRNA linear EST 12-MAY-2010 DEFINITION H3059D07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3059D07 3', mRNA sequence. ACCESSION BG067893 VERSION BG067893.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 810) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067893.1. Other_ESTs: H3059D07-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3059 row: D column: 07 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..810 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3059D07-3" /db_xref="taxon:10090" /clone="H3059D07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 161 a 224 c 220 g 205 t ORIGIN 1 attgcacatg gtccttttta ttttctgcag agaaaaggct tccttcctct gcagacctaa 61 agcctcaggc tggcagagtg acctcatcta gatgtgatca tgacgttaaa aagcattcct 121 gggtagtgat tttagacaga tggtgctgac atgaatgggc tcagagcagt aatggagggg 181 ggcaatgaat cacaggacag ctccaggcgt gttcccatta ccgctggatc aggacggaca 241 gggctgctga gaggtcccat gactccaatg tcttcgtggc gggaggagag ctcctggctg 301 aattcccgcc ttcggacatg tctggttgat ggcgagttgc tttcccgaga ggcttccctc 361 ccatcagttc ttcacatctc ccagacggag ctgggcaaag gaggtagcca gctgcagtgc 421 ttcctgaagg cagcccacgt tcttgcctgt ggcctgggcg gacatgtcct tgccaccgcc 481 tttaccatcc atcaggcctg aaacctgctg gacccactcg ctggctttca gtccccggtt 541 ggctgcattc tggggaacct ggcacaagca cgtgatcttg ccagcctcat tgtccaccgt 601 gaacagcatg gcagatgtct gaggagaatg cgtcttgaaa agcttcaacg cttcattcag 661 ggccttggcc gatgctccac tctccatctc caggatgacc agcggctggt tggggttgct 721 gtcaatcagc tgctttgtct tctctaacac tcgcttctgc acatcggctt tgctggcccg 781 gtctaggtca tccatgactt tcttcaggga //