LOCUS BG067646 687 bp mRNA linear EST 12-MAY-2010 DEFINITION H3056F07-3 NIA Mouse 15K cDNA Clone Set Mus musculus cDNA clone H3056F07 3', mRNA sequence. ACCESSION BG067646 VERSION BG067646.2 DBLINK BioSample: SAMN00163148 KEYWORDS EST. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. REFERENCE 1 (bases 1 to 687) AUTHORS Tanaka,T.S., Jaradat,S.A., Lim,M.K., Kargul,G.J., Wang,X., Grahovac,M.J., Pantano,S., Sano,Y., Piao,Y., Nagaraja,R., Doi,H., Wood,W.H. III, Becker,K.G. and Ko,M.S.H. TITLE Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (16), 9127-9132 (2000) PUBMED 10922068 COMMENT On Jan 26, 2001 this sequence version replaced BG067646.1. Other_ESTs: H3056F07-5 Contact: George J. Kargul Laboratory of Genetics National Institute on Aging/National Institutes of Health 333 Cassell Drive, Suite 4000, Baltimore, MD 21224-6820, USA Email: cdna@lgsun.grc.nia.nih.gov This clone set has been freely distributed to the community. Please visit http://lgsun.grc.nia.nih.gov/cDNA/15k.html for details. Plate: H3056 row: F column: 07 Seq primer: -21M13 Forward POLYA=Yes. FEATURES Location/Qualifiers source 1..687 /organism="Mus musculus" /mol_type="mRNA" /strain="C57BL/6J" /db_xref="niaEST:H3056F07-3" /db_xref="taxon:10090" /clone="H3056F07" /sex="clones arrayed from a variety of cdna libraries" /clone_lib="SAMN00163148 NIA Mouse 15K cDNA Clone Set" /dev_stage="Clones arrayed from a variety of cDNA libraries" /lab_host="DH10B" /note="Vector: pSPORT1; Site_1: SalI; Site_2: NotI; This clone is among a rearrayed set of 15,247 clones from 11 embryo cDNA libraries (including preimplantation stage embryos from unfertilized egg to blastocyst, embryonic part of E7.5 embryos, extraembryonic part of E7.5 embryos, and E12.5 female mesonephros/gonad) and one newborn ovary cDNA library. Average insert size 1.5 kb. All source libraries are cloned unidirectionally with Oligo(dT)-Not primers. References include: (1) Genome-wide expression profiling of mid-gestation placenta and embryo using a 15,000 mouse developmental cDNA microarray, 2000, Proc. Natl. Acad. Sci. U S A, 97: 9127-9132; (2) Large-scale cDNA analysis reveals phased gene expression patterns during preimplantation mouse develolpment, 2000, Development, 127: 1737-1749; (3) Genome-wide mapping of unselected transcripts from extraembryonic tissue of 7.5-day mouse embryos reveals enrichment in the t-complex and under-representation on the X chromosome, 1998, Hum Mol Genet 7: 1967-1978." BASE COUNT 200 a 136 c 141 g 210 t ORIGIN 1 aaattttatt tatattccca gaaagtcaac acatttattg tatgcagtca acagagtgga 61 acaatatcct catggaaggg tgcctccatt gtaacacact caaactacaa taatcctaga 121 gaagaaaaga gtgcttgata tgttttgcag atgcaacact catttacctg ctgtgaggat 181 gagcttgcct ttccagtgtg tctcagacat ttaggtaatt gacatatcca tgctcttttc 241 agcaatttca tcaatcccca atatcaagct tccatttctg tcttccacaa tcaatcgcac 301 agtgggtttt ttttcaaaaa agcaacattg gttctccaga tcataaacat aggacacacc 361 atacttagag catctggttg tagcaaaggt gacatcctct ggctgtctta tggccatgag 421 tatatccagg agttcagggc aaaaaatttc taatctgtga ggtagtatta catctctttt 481 atcatagcac tcctgagggg cagggtacag ttcaccaatt agctggctaa ggttggttgt 541 gtggtgtaga atttgtttca gggaaagcag agagagttta ttaccataga aattgacttc 601 tgcgagttgg taacattgac ttagggcagg tagcagggca ttgaagtgag tttcccccat 661 ttcacaggac ttcaattata ggcattc //